Anti-Lubricin/Proteoglycan 4; clone 9G3

Application

This Anti-Lubricin Antibody/Proteoglycan 4, clone 9G3 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of Lubricin/Proteoglycan 4.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Lubricin/Proteoglycan 4 in human liver and human heart tissue.
Immunohistochemistry Analysis: A representative lot detected Lubricin/Proteoglycan 4 in articular cartilage tissue (Elsaid, K.A, et al. (2012). Osteoarthritis and Cartilage. 20:940-948).
Immunohistochemistry Analysis: A representative lot detected Lubricin/Proteoglycan 4 in rat arterial cartilage tissue (Jay, G.D, et al. (2012). Arthritis & Rheumatism. 64(4):940-948).
Immunohistochemistry Analysis: A representative lot detected Lubricin/Proteoglycan 4 in articular cartilage from rat
(Manuscript in preparation).
Western Blotting Analysis: A representative lot detected Lubricin/Proteoglycan 4 in lubricin purified from synovial fluid of patients (Manuscript in preparation).

General description

The protein called Lubricin/Proteoglycan 4 is an important secreted protein in the joint critical for joint function and thus is also a focus of research in joint diseases as well. Lubricin/Proteoglycan 4 plays a role in boundary lubrication and prevents the deposition of cartilage from the synovial fluid by controlling the synovial growth of the synovial cells and inhibiting the adhesion of the cells to the cartilage surface. During development, Lubricin/Proteoglycan 4 also acts as a growth factor for hematopoietic and endothelial cell lineages, and Lubricin/Proteoglycan 4 appears important for proper barrier and lubrication development in tissues like the eye. Lubricin/Proteoglycan 4 is highly expressed in joints and near joint boundaries but can also be found in the brain, heart, lung, kidney and other tissues. Lubricin/Proteoglycan 4 has several isoforms that differ in their cell and tissue expression. Isoform C and D are the most widely expressed forms. Finally because of its effects on surfaces, Lubricin/Proteoglycan 4 is being examined in tissue engineering and artificial cartilage development as a treatment for joint diseases.

Immunogen

Recombinant protein corresponding to human Lubricin/Proteoglycan 4.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Format: Purified

Quality

Evaluated by Western Blotting in Lubricin purified protein.

Western Blotting Analysis: 0.02 µg/mL of this antibody detected Lubricin/Proteoglycan 4 in 1 µg of Lubricin purified protein.

Target description

~220 kDa observed. The calculated molecular weight of this protein is 151 kDa with many isoforms observed around 145 kDa and it can run higher due to glycosylation.